- The Effect of EDTA and Fetal Cord Serum Supplementation to Ham's F-10 Culture Medium on Development Potential of Mouse Embryos in Vitro
-
Byeong Seog Kim, Young GI Lee, Yoon Kee Park, Tae Hyung Lee, Sung Ho Lee
-
Yeungnam Univ J Med. 1995;12(1):124-134. Published online June 30, 1995
-
DOI: https://doi.org/10.12701/yujm.1995.12.1.124
-
-
 Abstract
 PDF
- It is the most important to select optimal culture conditions to promote safe embryo growth in the technique of human in vitro fertilization and embryo transfer. It has been shown that the addition of biologic fluids, such as blood serum, of various origins, improved fertilization and early cleavage rates in numerous species. The purpose of this study is to attempt to measure developmental potential of mouse eggs fertilized and cleaved in Ham's F10 culture medium containing a chelating agent, EDTA and fetal cord serum. In this study, we selected 40 female mice and 20 male mice, and investigated optimal serum concentration' for mouse embryo growth. Two cell stage mouse embryos were cultured in Ham's F-10 medium, Ham's F-10 medium with various concentrations of EDTA, or Ham's F-10 medium with EDTA and 10% human cord serum. Developmental ratios to morula in Ham's F-10 medium containing various concentrations of EDTA and/or 10% fetal cord serum were significantly higher than in unsupplemented Ham's F-10 medium (p<0.05). Developmental ratios to blastocyst in Ham's F-10 containing 10% fetal cord serum and 5011M or 100µM EDTA were significantly higher than in unsupplemented Ham's F-10 medium (p<0.05). Developmental ratios to morula in Ham's F-10 containing 10% fetal cord serum and 100µM EDTA were significantly higher than in Ham's F-10 with 10% fetal cord serum used commonly in many human IVF centers (p<0.05). Developmental ratio to blastocyst in Ham's F-10 containing 10% fetal cord serum and 100µM EDTA was significantly higher than in Ham's F-10 with 200µM EDTA (p<0.05). In summary, embryo development to morula and blastocyst was significantly higher in the presence of human cord serum or EDTA than in the unsupplemented medium. The most significantly development to morula and blastocyst was obtained at Ham’s F-10 medium with 100µM concentration of EDTA and 10% fetal cord serum. These results suggest that Ham’s F-10 medium containing 10% fetal cord serum and optimal concentrations of EDTA significantly promoted early cleavage of mouse zygotes, and these will be useful as basic data for the selection of culture medium in human in vitro fertilization.
- A case of Arnold-Chiari malformation.
-
Jong Ho Kim, Byeong Seog Kim, Mi Na Lee, Doo Jin Lee, Sung Ho Lee
-
Yeungnam Univ J Med. 1992;9(1):203-209. Published online June 30, 1992
-
DOI: https://doi.org/10.12701/yujm.1992.9.1.203
-
-
Abstract
PDF
- The Arnold-Chiari malformation (ACM) is an anomaly of the hindbrain consisting of two components: a variable displacement of a tongue of tissue derived from the inferior cerebellar vermis in the upper cervical canal and a similar caudal dislocation of the medulla and fourth ventricle. Hydrocephalus and meningomyelocele are another abnormalities that associated frequently. We present a case of Arnold-Chiari malformation with hydrocephalus and meningomyeolcele and a brief review of the literatures was added.
|
E-Submission
Yeungnam University College of Medicine
